The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A degree mutation was launched by the fast alter https://rosthornin-a66542.total-blog.com/about-sr-4835-51632062